Journal: International Journal of Molecular Sciences

Article Title: Antioxidant and Cytoprotective Effects of (−)-Epigallocatechin-3-(3″- O -methyl) Gallate

doi: 10.3390/ijms20163993

Figure Lengend Snippet: Protective effect of 3″Me-EGCG against ultraviolet B (UVB) irradiation. ( a ) HaCaT cells were pre-incubated with 3″Me-EGCG and UVB-irradiated as indicated. After 48 h, cell viability was examined by MTT assay. ( b ) Images of HaCaT cells after 3″Me-EGCG treatment and UVB irradiation. Images were captured using an optical microscope. ( c ) Whole lysate of UVB-irradiated HaCaT cells was for immunoblotting using phospho- or total antibodies against AKT1 and AKT2. β-actin was used as a loading control. ( d ) The cell viability of 3″Me-EGCG- or LY294002 (20 μM)-treated HaCaT cells in the presence of UVB was determined by MTT assay. ( e ) HA-AKT1, NF-κB-Luc, and β-galactosidase plasmids were transfected into HEK293T cells, and the cells were treated with 3″Me-EGCG (0–12.5 μM). NF-κB-mediated luciferase activity was measured by a luminometer. A β-galactosidase construct was used as a control. ## p <0.01 compared to normal group and * p < 0.05 and ** p < 0.01 compared to normal (untreated) or positive (induced) group. -: not treated and +: treated.

Article Snippet: Cells were irradiated with 30 mJ/cm 2 UVB (UVB lamp, Bio-link crosslinker BLX-312; Vilber Lourmat, Collegien, France).

Techniques: Irradiation, Incubation, MTT Assay, Microscopy, Western Blot, Control, Transfection, Luciferase, Activity Assay, Construct

Journal: Molecular and Clinical Oncology

Article Title: Narrow-band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells

doi: 10.3892/mco.2013.145

Figure Lengend Snippet: Effects of narrow-band UVB radiation on cytoskeletal F-actin in B16 melanoma cells. (A) Laser scanning confocal microscopy (LSCM) revealed that F-actin appeared organized in numerous clear stress fibers crossing the cytoplasm in non-irradiated cells. Following narrow-band UVB irradiation (100 mJ/cm 2 ), these stress fibers became obscure as F-actin was disassembled. This event could be observed as early as (B) 30 min, becoming more evident at (C) 60 min, with the appearance of punctate spots at (D) 6 h.

Article Snippet: The cells were seeded in 96-well plates at a density of 1×10 4 cells/well and incubated for 24 h. Subsequently, the cells were exposed to various doses of narrow-band UVB radiation (0, 25, 50, 100, 200 and 300 mJ/cm 2 ) and were incubated for an additional 24 h. Cell viability was assessed using a commercially available kit (Cell Counting kit-8; Dojindo Co., Ltd., Kumamoto, Japan) according to the manufacturer’s instructions to determine the appropriate dose of narrow-band UVB radiation for the subsequent experiments.

Techniques: Confocal Microscopy, Irradiation

Journal: Molecular and Clinical Oncology

Article Title: Narrow-band UVB radiation promotes dendrite formation by activating Rac1 in B16 melanoma cells

doi: 10.3892/mco.2013.145

Figure Lengend Snippet: Effects of narrow-band UVB radiation on the activity of GTP-RhoA and -Rac1 in B16 melanoma cells. (A) The pull-down analysis revealed that the GTP-Rac1 protein expression was significantly increased at 15 min and by 2-fold at 30 min following narrow-band UVB irradiation (100 mJ/cm 2 ), followed by a minor decrease, although the protein levels remained elevated at 60 and 120 min compared to control cells. (B) GTP-RhoA levels were marginally changed during the first 30 min after irradiation, were elevated at 60 min and by 1.6-fold at 120 min.

Article Snippet: The cells were seeded in 96-well plates at a density of 1×10 4 cells/well and incubated for 24 h. Subsequently, the cells were exposed to various doses of narrow-band UVB radiation (0, 25, 50, 100, 200 and 300 mJ/cm 2 ) and were incubated for an additional 24 h. Cell viability was assessed using a commercially available kit (Cell Counting kit-8; Dojindo Co., Ltd., Kumamoto, Japan) according to the manufacturer’s instructions to determine the appropriate dose of narrow-band UVB radiation for the subsequent experiments.

Techniques: Activity Assay, Expressing, Irradiation

Journal: Marine Drugs

Article Title: Inhibition of Ultraviolet B-Induced Expression of the Proinflammatory Cytokines TNF-α and VEGF in the Cornea by Fucoxanthin Treatment in a Rat Model

doi: 10.3390/md14010013

Figure Lengend Snippet: Experimental protocol for UVB irradiation and fucoxanthin treatment. Daily UVB irradiation (indicated by arrows) was performed from day 1 to day 5. The UVB/vehicle group served as UVB controls exposed to UVB light and pretreated with an intravenous injection of 0.1% dimethyl sulfoxide solution mixed with 0.1 mL PBS daily for seven days (day 0 to day 6). UVB/fucoxanthin groups were exposed to UVB irradiation and pretreated intravenously with 0.5 or 5 mg/kg fucoxanthin daily (indicated by green point) for seven days (day 0 to day 6).

Article Snippet: After anesthesia with intraperitoneal injection of sodium pentobarbital (60 mg/kg body weight), the eyes were exposed to 550 μW/cm 2 of UVB light (UVM-28; UVP Inc., San Gabriel, CA, USA) for 3 min in a darkroom.

Techniques: Irradiation, Injection